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The proteins ZO 1 and occludin, critical components of tight junctions, were analysed next (summarized in Table 2). ZO 1 and Occludin were observed not only in the membrane but also in the cytoplasm of half of the cell lines (COGA 2, COGA 3, COGA 8 and COGA 10). ZO 1 was detected in considerable concentrations in the nuclei of COGA 8 cells (see Table 2).
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