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The proteins ZO 1 and occludin, critical components of tight junctions, were analysed next (summarized in Table 2). ZO 1 and Occludin were observed not only in the membrane but also in the cytoplasm of half of the cell lines (COGA 2, COGA 3, COGA 8 and COGA 10). ZO 1 was detected in considerable concentrations in the nuclei of COGA 8 cells (see Table 2).

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Sandra Skelly lives with diabetes and is always looking for good low or no sugar desserts. She finds that not all recipes can be adapted to sugar substitutes like Splenda, but some can, including this recipe for warm berry sauce. And Sue Feldman sent in a sugarless recipe for peanut butter brownie cookies, also made with Splenda.

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